· 姓名:林凌

· 职称:副教授

· 电话:0553-3869297

· 邮箱:linling8@ahnu.edu.cn

◆主要学习工作经历

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· 1999.9 - 2003.6,华中农业大学生命科学与技术学院,生物工程学士学位;

· 2003.9 - 2009.6,华中农业大学生命科学与技术学院,微生物学博士学位;

· 2009.7 - 2012.6,安徽师范大学生命科学学院,讲师;

· 2017.5 - 2017.8,国外访问学者;

· 2012.7至今,安徽师范大学生命科学学院,副教授。

◆主要社会兼职

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· 中国微生物学会会员

· 中国遗传学会

· 广东企业科技特派员

◆主要研究内容

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· 生化酶的分子生物学活性与功能研究

· 微生物代谢工程制备药物中间体

· 工业微生物技术

◆主要研究课题

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· 安徽省高等学校自然科学研究项目. 青海地区适冷纤维素酶的筛选及其催化机制探索(No. KJ2017A321),2017-2018,主持.

· 安徽省自然科学基金项目. 空间结构进化改良纤维素酶催化活性(No. 1208085QC56),2012-2014,主持.

· 安徽师范大学生命科学学院重点实验室与重点学科开放基金项目. 纤维素酶活性的分子定向进化,2010,主持.

· 安徽师范大学科研培育基金项目. 纤维素酶定向进化,2011,主持.

· 浙江长兴制药有限公司研发项目. 生物转化法制备阿魏酸生产工艺开发,2011-2012,主持.

· 广东省动物药品(容大)工程技术研究中心协同创新与平台环境建设项目. 硫酸黏菌素生物发酵生产工艺精进研究,2014-2015,主持.

◆主要研究成果

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1、代表性论文(20篇以内,*号为通讯作者):

· Mingming Chen, Na Qin, Wenqin Pei, Qianhui Li, Qing Yang,Yao Chen, Duntao Huang, Yingying Xiang, Ling Lin*. Draft Whole-Genome Sequences of Zhihengliuella halotolerans La12 and Microbacterium kitamiense Sa12, Strains with Cellulase Activity, Isolated from the Qinghai-Tibetan Plateau. Genome Announc 2018, 6e01531-17.

· Ling Lin*, Xiaozhou Liu, Yating Zhou, Linyan Guan, Jiajia He, Weiqian Huang. A Novel pH-Stable, Endoglucanase (JqCel5A) isolated from a Salt-lake Microorganism, Jonesia quinghaiensis. Electronic Journal of Biotechnology (ISSN: 0717-3458). 24(2016):56-62.

· Ling Lin*, Chenggen Fu, Weiqian Huang. Improving the activity of the endoglucanase, Cel8M from Escherichia coli by error-prone PCR. Enzyme and Microbial Technology (2016). 86:52-58.

· 何佳佳,关琳燕,刘小舟,周亚婷,文习东,林 凌*. 一株阿里地区产纤维素酶菌株的筛选与鉴定. 中国酿造. 2016, 35(11):64-68.

· 金朝晖,陈琼,赵树楠,林凌*.一株高寒地区产纤维酶细菌的筛选、鉴定及酶学性质研究. 中国酿造. 2015, 7(7):65-68.

· 张瑾,张虹,王玲,林凌*. 一种筛选纤维素酶产生菌的改良方法. 中国酿造. 201332(7):101-103.

· 陆宁,柳帅,张玉洁,林凌*. 枯草芽孢杆菌内切葡聚糖酶Ala263位点突变对其活性的影响. 中国酿造. 201332(3):122-125.

· Ling Lin*, Xianzhao Kan, Hao Yan, Danni Wang.  Characterization of extracellular cellulose-degrading enzymes from Bacillus thuringiensis strains. Electronic Journal of Biotechnology, (2012), 15(3):fulltext-1.

· Lin L, Meng X, Liu P, Hong Y, Wu G, Huang X, Li C, Dong J, Xiao L, Liu Z. Improved Catalytic Efficiency of Endo-β-1, 4-glucanase from Bacillus subtilis BME-15 by Directed Evolution. (2009) Appl Microbiol Biotechnol. 82 (4): 671-679

· Liu P, Hong Y, Lin Y, Fu X, Lin L, Li C, Meng X, Liu Z. A frequency-controlled random mutagenesis method to aim specially at GC-rich gene. (2009) Anal Biochem. 388(2):356-358.

· Li C, Hong Y, Shao Z, Lin L, Huang X, Liu P, Wu G, Meng X, Liu Z. A novel alkali-stable, cellulase-free xylanase from deep-sea Kocuria sp. Mn22. (2009) J Microbiol Biothechn. 19(9):873-880.

· Meng X, Shao Z, Hong Y, Lin L, Li C, Liu Z. A novel pH-stable, bifunctional xylanase isolated from a deep-sea microorganism, Demequina sp. JK4. (2009) J Microbiol Biothechn. 19(10):1077-1084.

· Fu X, Liu P, Lin L, Hong Y, Huang X, Meng X, Liu Z. A novel endoglucanase (Cel9P) from a marine bacterium Paenibacillus sp. BME-14. (2009) Appl Biochem Biotech. 160(6):1627-1636, 2010

· Gaobing Wu, Chunfang Feng, Yuzhi Hong , Aizhen Guo, Sha Cao, Junli Dong, Ling Lin, Ziduo Liu. Soluble expression and purification of the protective antigen in E. coli and identification of a novel dominant-negative mutant N435C.2010Applied Microbiology and Biotechnology. 87(2):609-616

· Xiaoluo Huang, Zongze Shao, Yuzhi Hong, Ling Lin, Chanjuan Li, Fei Huang, Hui Wang, Ziduo Liu. Cel8H, a novel endoglucanase from the halophilic bacterium Halomonas sp. strain S664: molecular cloning, heterogonous expression and biochemical characterization.2010The Journal of Microbiology. 48(3):318-324.

· Fu, Xiaoyu, Xiaoluo Huang, Pengfu Liu, Ling Lin, Gaobing Wu, Chanjuan Li, Chunfang Feng, and Yuzhi Hong. Cloning and Characterization of a Novel Mannanase from Paenibacillus sp. BME-14. (2010J Microbiol Biothechn. 20(3):518-524.

2、专利:

· 林凌,王俊政,陈剑,付成根. 一种生物法生产阿魏酸工程菌株及其构建方法. 专利号:CN 103820375 B.